This environment rich in oil could be the source of proliferation of hydrocarbonoclastic bacteria. Most hydrocarbonoclastic bacteria in the total environment are diazotrophic. Most hydrocarbonoclastic bacteria in the total environment are diazotrophic, which highlights their value in the bioremediation of hydrocarbon contaminants. In marine coastal sediments, mohcb are particularly subjected to extreme fluctuating conditions due to redox oscillations several times a day as a result of. Newtown creek is an industrial waterway and former tidal wetland in new york city.
Frontiers crossbioaugmentation among four remote soil. The results showed that crossbioaugmentation did not bring about reproducible effects on oil. Most hydrocarbonoclastic bacteria in the total environment. Crossbioaugmentation was done among them, and the oilconsumption and the constituent communities of hydrocarbonoclastic bacteria were monitored periodically through 6 months. Thus, for example, in contrast to the culturedependent technique, the primers used in the molecular approach preferentially amplified the 16s rdnas of hydrocarbonoclastic bacteria in total genomic dnas of all the studied environmental samples, and in addition, failed to reveal in any environmental sample members of the actinobacteria. The position of the isolated strains among closely related members from database is depicted in the figure. The present study focused on isolation, molecular identification, morphological and biochemical characterization of bacteria possessing hydrocarbondegrading properties. Therefore, such microorganisms are continuously discharged with feces, and e. View enhanced pdf access article on wiley online library html view download pdf for offline viewing.
Deciphering the ecology of marine obligate hydrocarbonoclastic bacteria mohcb is of crucial importance for understanding their success in occupying distinct niches in hydrocarboncontaminated marine environments after oil spills. The dominance of aerobic hydrocarbonoclastic bacteria in these tidal biofilms suggests that they are hot spots of hydrocarbon degradation in coastal wetlands which could serve to transport primed hydrocarbondegrading communities to other parts of the marsh and further afield. We further discuss briefly the production and export of lipophilic compounds by bacteria belonging to the alcanivorax genus, which became a model strain of an unusual group of obligate hydrocarbonoclastic bacteria ohcb and discuss the possibility to produce neutral lipids using a. Characterization of naphthalenedegrading bacteria isolated from. Total heterotrophic bacterial counts ranged from 0. Petroleum microbiology is a branch of microbiology that deals with the study of microorganisms that can metabolize or alter crude or refined petroleum products. Oxygen content is a limiting factor in the process of hydrocarbon compounds degradation by hydrocarbonoclastic bacteria. While pseudoxanthomonas and alcanivorax were frequently recorded in the literature as hydrocarbonoclastic bacteria 27,33, nothing is known so far.
This study identified bacterial strains that can potentially remediate the. Phylogenetic tree of hydrocarbonoclastic bacteria isolated from crude oil contaminated soil of assam. Efficacy of intervention strategies for bioremediation of. Hackbridge railway station, a railway station in south london. This disambiguation page lists articles associated with the title hcb. Bacterial diversity impacts as a result of combined sewer. Characterization of furnace oil bioremediation potential. The isolates were inoculated separately into mineral salt medium supplemented with 1% crude oil to determine their. Coliform bacteria belong to the family enterobacteriaceae, which comprises facultative anaerobic bacteria naturally inhabiting digestive tracts of warmblooded animals including man. Isolation and identification of hydrocarbon degradation bacteria. Identify and describe culture media for the growth of bacteria, including examples of allpurpose media, enriched, selective, differential, defined, and enrichment media.
Alcanivorax is an important member of the hydrocarbonoclastic group known for. In this work, biofilms containing hydrocarbonoclastic bacteria were successfully established on glass slides by submerging them in oilfree and oil. Three bacterial strains were isolated from various crude oil contaminated environments and characterized using standard identification techniques. Dcpip test is a quick and reliable method for screening of hydrocarbonoclastic bacteria. Visualisation of the obligate hydrocarbonoclastic bacteria polycyclovorans algicola and algiphilus aromaticivorans in cocultures with microalgae by cardfish. Crude oilinduced structural shift of coastal bacterial communities of rod bay terra nova bay, ross sea, antarctica and characterization of cultured coldadapted hydrocarbonoclastic bacteria. Coliform bacteria for bioremediation of waste hydrocarbons. Microbial biodegradation is the use of bioremediation and biotransformation methods to harness the naturally occurring ability of microbial xenobiotic metabolism to degrade, transform or accumulate environmental pollutants, including hydrocarbons e. Hydrocarbons are principle constituents of petroleum, related byproducts like coal tar, diesel, petrol, gasoline. Pdf marine hydrocarbonoclastic bacteria and their function in. Using biomolecular methods, five distinct species of hydrocarbonoclastic marine bacteria and two species likely belonging to the bacillus genera were identified.
Growth rates increased with increasing alkane chain length up to a. Characterization of hydrocarbonoclastic bacterial communities from mangrove sediments in guanabara bay, brazil. This study aims to to determine the optimal agitation speed for batch fermentation process by hydrocarbonoclastic bacteria isolated from the waste tank of pt. We compared these obligately thermophilic hydrocarbonoclastic bacteria by determing the electrophoretic patterns of enzymes and soluble proteins and by using deoxyribonucleic aciddeoxyribonucleic acid hybridization techniques. Pdf characterization of hydrocarbonoclastic bacterial. Hydrocarbonoclastic bacteria are ubiquitous and evolutionarily old lineages that have adapted to oil components released at natural oil seeps 26, 27. Bioremediation potentials of hydrocarbonoclastic bacteria. Accumulation of storage lipids, serving as endogenous carbon and energy sources during starvation periods, might be a potential adaptation mechanism for coping with nutrient limitation, which is a frequent stress factor. Ability of the socalled obligate hydrocarbonoclastic bacteria to. Marine life in the mesopelagic zone 200 to m is subjected to. Petroleum effluent is discharged into river rido and this poses a threat to the communities that utilize the water downstream of river rido, the ultimate recipient of the effluent.
Specialized hydrocarbonoclastic bacteria prevailing in seawater. Phytoplankton have been shown to harbour a diversity of hydrocarbonoclastic bacteria hcb, yet it is not understood how these phytoplankton. There is little information on how different strategies for the bioremediation of marine oil spills influence the key indigenous hydrocarbon. Marine hydrocarbonoclastic bacteria and their function in. Pseudomonas aeruginosa is a common encapsulated, gramnegative, rodshaped bacterium that can cause disease in plants and animals, including humans. This investigation was carried out to characterize microbial communities in selected crude oil polluted sites in bodo community, gokana local government area of rivers state, nigeria. Pearsons productmoment correlation analysis of the relationships between total hydrocarbon content thc and hydrocarbon utilizing bacteria population hub in mangrove sediments and overlying water of the qua iboe estuary, nigeria was carried out. Pdf eightytwo out of the 100 hydrocarbonoclastic bacterial species that have been already isolated from oilcontaminated kuwaiti.
Typically, their population size in the absence of oil spills is. Deoxyribonucleic acid homology and other comparisons among. Hydrocarbonoclastic bacteria related publications 1 density of hydrocarbonoclastic bacteria and polycyclic aromatic hydrocarbon accumulation in iko river mangrove ecosystem, nigeria authors. Culturable hydrocarbonoclastic marine bacterial isolates. The study also aimed at optimizing appropriate culture conditions for the isolates as well as screening for alkane hydroxylase enzyme. Marine hydrocarbonoclastic bacteria published by woodhead publishing limited, 20 energetic and biosynthetic metabolism. Potential hydrocarbonoclastic bacteria biotech articles.
We concluded that most hydrocarbonoclastic bacteria are diazotrophic, which allows for their wide distribution in the total environment. The objective of the study is to isolate and identify indigenous bacterial strains from marine waters of two ports in the eastern algerian coast and to test their growth in the presence of. For over a century, organic compounds, heavy metals, and other forms of industrial pollution have disrupted the creeks environment. Selective substrate utilization by marine hydrocarbonoclastic bacteria selective substrate utilization by marine hydrocarbonoclastic bacteria soli, giorgio. Enimie endurance oaikhena 1, dominic bawa makaije 1, samuel denwe 1, muhammad muktar namadi 2, omolola elizabeth fatanmi 1. Mwpb18 and pseudomonas fluorescens owpb17 demonstrated commendable protease production ability in skimmed milkminimal medium. Manual of industrial microbiology and biotechnology, second edition. However, investigations of growth behavior and physiology of these bacteria when cultivated with n alkanes of different chain lengths c6 to c30 as the substrates are still lacking. For example, if we want to isolate bacteria that break down crude oil, hydrocarbonoclastic bacteria. Download fulltext pdf download fulltext pdf specialized hydrocarbonoclastic bacteria prevailing in seawater around a port in the strait of malacca article pdf available in plos one 86.
Soil samples were collected from kuwait, lebanon, egypt, and germany, and artificially polluted with 3% ww crude oil. Crude oil is toxic to metazoan life yet is a supply of extremely energyrich carbon sources for hydrocarbonoclastic bacteria. Application of correlation analysis in assessment of. In organic chemistry, an alkane, or paraffin a historical name that also has other meanings, is an acyclic saturated hydrocarbon. Pdf hydrocarbonoclastic microorganisms elaborate a number of hydrocarbons utilising genes that enable them to use crude oil hydrocarbons as carbon. A method for removal of freefloating oil from an aqueous environment by biodispersion and bioutilization comprising the steps of. If an internal link led you here, you may wish to change the link to point directly to the intended article. The mechanism by which hydrocarbonoclastic bacteria degrade hydrocarbons will also be discussed. A total of five hydrocarbonoclastic bacteria were isolated from the petroleum refinery effluent. Release of petroleum oil and its products into the environment is a worldwide concern. Sequencing of the 16s rrna gene showed that most isolates belong to genera of hydrocarbonoclastic bacteria alcanivorax, generalist hydrocarbons degraders marinobacter, pseudomonas, gordonia, halomonas, erythrobacter and brevibacterium and other bacteria not known as hydrocarbon degraders xanthomarina but were. Water soluble fraction of crude oil uncouples protease. Specialized hydrocarbonoclastic bacteria shcb, which use limited carbon sources with a preference for petroleum hydrocarbons, were found.
Gelatinizing oil in water and its removal via bacteria. Attempts to establish hydrocarbonoclastic biofilms that could be applied in wastehydrocarbon removal are still very rare. Native hydrocarbonoclastic bacteria and hydrocarbon. Isolation and characterization of gram negative hydrocarbonoclastic bacteria and fungi strains from noncontaminated, premium motor spirit pms contaminated and refuse environment. African journal of environmental science and technology. Characterization of furnace oil bioremediation potential of hydrocarbonoclastic bacteria isolated from petroleum contaminated sites of the sundarbans, bangladesh. The marine hydrocarbonoclastic bacterium alcanivorax borkumensis is able to degrade mixtures of n alkanes as they occur in marine oil spills. Abstract the main purpose of the study was to isolate strains of bacteria capable of degrading hydrocarbons from contaminated mangroves and to investigate the ability of the isolated bacteria to degrade total petroleum hydrocarbons tph in a microcosm model of an oily sludge. Pdf isolation and characterization of gram negative. Oxygen may be supplied through agitation stirring during fermentation process by hydrocarbonoclastic bacteria. Alawadhiability of the socalled obligate hydrocarbonoclastic bacteria to. Screening and characterization of hydrocarbonoclastic. Petroleum, crude oil or oil and hydrocarbons are sometimes used synonymously.
On the other hand, these bacteria only grew and consumed crude oil in the nano 3free medium when the top space gas contained nitrogen. Hydrocarbonoclastic means degrading hydrocarbons or simply eating or breaking the hydrocarbon molecules. Bioremediation potentials of hydrocarbonoclastic bacteria isolated from petroleum refinery effluent. The results show that there was in general a close relationship between the two variables, but also a large amount of variation not explained. Evaluation, characterization and adaptation of hydrocarbonoclastic bacteria. Hydrocarbonoclastic bacteria, a form of oil biodegrader. Bacterial strains in our culture collection similar to the recently proposed species thermoleophilum album were examined for generic relatedness. Pdf specialized hydrocarbonoclastic bacteria prevailing. Narjes dashti, 1 nedaa ali, 1 mohamed eliyas, 1 majida khanafer, 1 naser a. Pdf most hydrocarbonoclastic bacteria in the total environment. Characterization and assessment of biosurfactant producing. The algerian coastline is being exposed to several types of pollution, including that of hydrocarbons. Marine hydrocarbonoclastic bacteria and their function in bioremediation of marine oil spills.
The bioremediation potential of hydrocarbonoclastic. The present study involves the characterization and assessment of biosurfactant producing indigenous hydrocarbonoclastic bacteria and their potential application in bioremediation processes. Usually, in the aerobic metabolism, activation of hydrocarbons is mediated by the addition of one or two oxygen atoms which provide reactive molecules for subsequent reactions. They are called hydrocarbonoclastic bacteria and play a key role in the removal of hydrocarbons from polluted environments harayama et al. Effect of agitation to hydrocarbon degradation by a. Central role of dynamic tidal biofilms dominated by. Three hydrocarbonoclastic bacteria isolated from crude oilimpacted mesotidal waters of the niger delta area of nigeria, and identified, by 16s rrna sequencing, as vibrio fluvialis owpb63, serratia sp. Specialized hydrocarbonoclastic bacteria prevailing in. Enhanced crude oil biodegradative potential of natural.
Banat im, daffonchio d, boon n and borin s 2016 hydrocarbonoclastic alcanivorax isolates exhibit different physiological and expression responses to ndodecane. Download pdf accepted author manuscript, 437 kb licence. The potential use of these bacteria strains as environmental cleanup agents was tested by culturing them with six. It is one of the most polluted water bodies in the united states and was designated as a superfund site in 2010. Bergeys manual of systematic bacteriology, 2nd edn, vol. These microorganisms, also called hydrocarbonoclastic microorganisms, can degrade hydrocarbons and, include a wide distribution of bacteria, methanogenic. Marine hydrocarbonoclastic bacteria, like alcanivorax borkumensis, play a globally important role in bioremediation of petroleum oil contamination in marine ecosystems. Us6267888b1 biodispersion as a method for removal of. Two bacterial strains jc109t and jc261 were isolated from a sediment sample collected from a shrimp cultivation pond in tamil nadu india.
116 412 239 850 636 963 149 482 1209 1580 1038 1259 843 1083 613 487 1406 225 1077 709 254 149 260 1541 1140 848 373 1089 849 374 1068 967 248 929 377 708 1176 588 1141 357 723 527 65 630 1487 915 115